A simple, precise, accurate, cost effective stability indicating UV Spectrophotometric method has been developed for the estimation of Methotrexate shows highest ?max at 303nm. Beer's law (linearity response) was found over a concentration range of 2-10 µg /mL with good correlation coefficient (r2 = 0.9987 and the values of standard deviation were satisfactory low and the recovery studies were close to 100%. The Proposed spectrophotometric method was validated as per the ICH Q1A (R2) guidelines. Hence this method can be safely be employed for the routine quality control analysis of Methotrexate.
Methotrexate, Method Development, UV- Visible Spectroscopy
International Journal of Trend in Scientific Research and Development - IJTSRD having
online ISSN 2456-6470. IJTSRD is a leading Open Access, Peer-Reviewed International
Journal which provides rapid publication of your research articles and aims to promote
the theory and practice along with knowledge sharing between researchers, developers,
engineers, students, and practitioners working in and around the world in many areas
like Sciences, Technology, Innovation, Engineering, Agriculture, Management and
many more and it is recommended by all Universities, review articles and short communications
in all subjects. IJTSRD running an International Journal who are proving quality
publication of peer reviewed and refereed international journals from diverse fields
that emphasizes new research, development and their applications. IJTSRD provides
an online access to exchange your research work, technical notes & surveying results
among professionals throughout the world in e-journals. IJTSRD is a fastest growing
and dynamic professional organization. The aim of this organization is to provide
access not only to world class research resources, but through its professionals
aim to bring in a significant transformation in the real of open access journals
and online publishing.