The aqueous extract of Punica granatum L. peel was prepared by basic one-step liquid-liquid extraction. The extraction method involved partitioning 10% v/v water in methanol extract of the peel between ethyl acetate & 2% aqueous acetic acid. This specific extraction process increases the ellagic acid content by approximately double w/w. furthermore, the antioxidant activity is evaluated by the DPPH, radical scavenging assay, which tends to increase ED50 roughly by triple the value µg/ml. these stability evaluations were measured under several conditions & temperatures, over a couple of months with successful results. The factors like humidity, sunlight, temperature fluctuations etc were all experimented during the stability test. Whereas, the acidity (pH) of the extract was the only loop-hole making it unstable in the aqueous or syrup (semi-solid) condition. Hence, the chemical constituent in the extract was stabilized when the extract was kept in amorphous, dried (powder) state.
punica granatum L., ellagic acid, DPPH, peel extract
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