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Molecular Approaches for Detection of Pebrine Disease in Sericulture

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Molecular Approaches for Detection of Pebrine Disease in Sericulture


Ayantika Bhardwaj | Ashok Kr. Thakur



Ayantika Bhardwaj | Ashok Kr. Thakur "Molecular Approaches for Detection of Pebrine Disease in Sericulture" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-8 | Issue-3, June 2024, pp.986-988, URL: https://www.ijtsrd.com/papers/ijtsrd66036.pdf

Nosema bombycis is an obligate intracellular parasite that causes pebrine disease by infecting Bombyx mori. The disease is transmitted transovarially and spreads rapidly in silkworm seed multiplication farms. Pebrine is the only quarantine requirement in silkworm egg production and is usually monitored by mother insect examination and light microscopy for specific spores. Practically spore detection is a bit technical, laborious and the life cycle stages of N. bombycis can also be observed by microscopy. However, routine monitoring of pebrine disease through light microscopy is limited by specificity and sensitivity. More practical and feasible diagnostic methods for pebrine monitoring have emerged with the development of immunodiagnostic and nucleic acid based detection techniques. Immunodiagnostic assays such as latex agglutination and ELISA using polyclonal and monoclonal antibodies were developed for diagnosis and characterization of microsporidian infections. However, false positives and low-level infections made immunoassays less reliable. Nucleic acid-based detection methods using DNA sequences specific for N. bombycis can detect particularly low levels of infection. Major DNA diagnostics for detecting N. bombycis include PCR, loop-mediated isothermal amplification (LAMP) and lateral flow assays that are high-throughput and quantitative, as well as capable of detecting multiple diseases simultaneously. Molecular diagnostic techniques are more advantageous than microscopy because of increased sensitivity, specificity, rapid and easy result interpretations. The molecular diagnostic tests described for the identification of microsporidia infecting mulberry silkworm are given in this chapter.

Nosema bombycis, pebrine, diagnostic, detection, silkworm etc.


IJTSRD66036
Volume-8 | Issue-3, June 2024
986-988
IJTSRD | www.ijtsrd.com | E-ISSN 2456-6470
Copyright © 2019 by author(s) and International Journal of Trend in Scientific Research and Development Journal. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0) (http://creativecommons.org/licenses/by/4.0)

International Journal of Trend in Scientific Research and Development - IJTSRD having online ISSN 2456-6470. IJTSRD is a leading Open Access, Peer-Reviewed International Journal which provides rapid publication of your research articles and aims to promote the theory and practice along with knowledge sharing between researchers, developers, engineers, students, and practitioners working in and around the world in many areas like Sciences, Technology, Innovation, Engineering, Agriculture, Management and many more and it is recommended by all Universities, review articles and short communications in all subjects. IJTSRD running an International Journal who are proving quality publication of peer reviewed and refereed international journals from diverse fields that emphasizes new research, development and their applications. IJTSRD provides an online access to exchange your research work, technical notes & surveying results among professionals throughout the world in e-journals. IJTSRD is a fastest growing and dynamic professional organization. The aim of this organization is to provide access not only to world class research resources, but through its professionals aim to bring in a significant transformation in the real of open access journals and online publishing.

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