The article takes into an account of the extraction of different types of biochemical analysis of Pigeon pea with an insecticide (Tafgor) in a laboratory condition. Pigeon pea is a perennial tropical legume grown mainly in India. It belongs to the family Fabaceae. More than any other legumes adapted to the region, Pigeon pea uniquely combines optimal nutritional profiles and high tolerance to environmental stresses, high biomass productivity. Environmental stresses play a crucial role in the productivity, survival and reproductive biology of plants and crops. They result in diminish and loss of crop production. Dimethoate is a widely used organophosphate and acaricide. TAFGOR insecticide (Dimethoate), used as a test chemical, was guaranteed at reagent from Rallis limited, India. First stock solution was prepared by dissolving 1g. of test chemical in 1L of distilled water. we have chosen only 8 concentrations (control,50,100,200,400,600,800,1000) for biochemical analysis. This experiment is done under laboratory conditions with sowing 10 seeds in 8 germination bowls which contain 200 gm. of soil and sand (3:1). The following day, after the exposure of seeds to light, there is rapid elongation of radicle and plumule. The percentage of amino acid content in root and shoot increases at 200 PPM and then decreases at 1000 PPM. The percentage of sugar in root and shoot increases at 400 PPM but decreases at 1000 PPM is 204.70%.and 51.71%. The percentage of protein of shoot is at 400 PPM increases but at 1000 PPM it decreases up to 56.81%. Similarly, in root the percentage of protein decreases at 200 PPM and at 1000 PPM it is 52.94%. At 200 PPM and 1000 PPM the percentage of DNA are 822.99% and 461.38% respectively. Similarly, in root, the percentage of DNA at 200 PPM is 373.42% and at 1000 PPM, it is 209.35%. Due to positive effect, at first it increases to the 200 PPM and after that it decreases due to more toxic effect. At 200 PPM and 1000 PPM the percentage of RNA are 117.01% and 96.35% respectively. Similarly, in root the percentage of RNA is 121.25% and at 1000 PPM it is 82.72%. Due to positive toxicity at first it increases to the 200 PPM and after that it decreases due to more toxic effect. The percentage of the biochemical contents increases from control to 200 or 400 PPM and then the percentage becomes lesser due to more toxic effect.
BIOCHEMICAL PARAMETERS, INSECTICIDE, Cajanus cajan L., TAFGOR
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