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Cost-effective Analysis of Different Soil Samples to Check their Potential for Producing Cellulose Degrading Bacteria

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Cost-effective Analysis of Different Soil Samples to Check their Potential for Producing Cellulose Degrading Bacteria


Khushboo Pal | Anshika Sharma | Tanu Sharma | Dr. Divya Sharma



Khushboo Pal | Anshika Sharma | Tanu Sharma | Dr. Divya Sharma "Cost-effective Analysis of Different Soil Samples to Check their Potential for Producing Cellulose Degrading Bacteria" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-5, August 2021, pp.799-804, URL: https://www.ijtsrd.com/papers/ijtsrd43937.pdf

The present investigation was undertaken to isolate and screen the cellulase producing bacteria from soil. Cellulose is considered to be the most abundant carbohydrate on earth along with hemicelluloses and lignin, which constitutes the plant cell wall. Cellulolytic enzymes are the backbone of various industries including food, animal feed, brewing, wine, agricultural biomass refining, pulp, paper, textile and ethanol production. In this paper, cellulose degrading bacteria was isolated and screened from different soil samples (from agriculture farm, waste dumping area and termite soil) using Carboxymethyl cellulose (CMC), wheat bran and waste paper plus nutrient agar as a selective medium. After screening, only five isolates (KHU2, KHU8, KHU9, KHU13 and KHU14) were selected due to the maximum diameter of zone of substrate hydrolysis. The diameter of zone of substrate hydrolysis in NAM plates containing wheat bran for the isolates KHU2, KHU8, KHU9, KHU13 and KHU14 was 8±3mm, 21±2mm, 13±1mm, 12±1mm and 10±2mm, respectively, whereas the diameter of zone of substrate hydrolysis in NAM plates containing waste paper for the isolates KHU2, KHU8, KHU9, KHU13 and KHU14 was 13±1mm, 24±1mm, 17±1mm, 14±2mm and 15±1mm respectively. This was compared with the diameter of zone of substrate hydrolysis in NAM plates containing CMC cellulose was also measured for the isolates KHU2, KHU8, KHU9, KHU13 and KHU14 was 16±1mm, 25±1mm, 22±1mm, 19±2mm and 20±2mm. It was concluded that the diameter of zone of substrate hydrolysis on waste paper was nearly same as obtained with CMC cellulose and the highest diameter of zone of substrate hydrolysis was seen in isolate KHU8 (25±1mm) in NAM plates plus CMC cellulose from termite soil sample. So, termite soil has the maximum potential to produce cellulose degrading bacteria.

Cellulose; cellulase; isolation; screening; wheat bran; waste paper; CMC


IJTSRD43937
Volume-5 | Issue-5, August 2021
799-804
IJTSRD | www.ijtsrd.com | E-ISSN 2456-6470
Copyright © 2019 by author(s) and International Journal of Trend in Scientific Research and Development Journal. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0) (http://creativecommons.org/licenses/by/4.0)

International Journal of Trend in Scientific Research and Development - IJTSRD having online ISSN 2456-6470. IJTSRD is a leading Open Access, Peer-Reviewed International Journal which provides rapid publication of your research articles and aims to promote the theory and practice along with knowledge sharing between researchers, developers, engineers, students, and practitioners working in and around the world in many areas like Sciences, Technology, Innovation, Engineering, Agriculture, Management and many more and it is recommended by all Universities, review articles and short communications in all subjects. IJTSRD running an International Journal who are proving quality publication of peer reviewed and refereed international journals from diverse fields that emphasizes new research, development and their applications. IJTSRD provides an online access to exchange your research work, technical notes & surveying results among professionals throughout the world in e-journals. IJTSRD is a fastest growing and dynamic professional organization. The aim of this organization is to provide access not only to world class research resources, but through its professionals aim to bring in a significant transformation in the real of open access journals and online publishing.

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