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Isolation and Identification of Bacteria from Peeled and Ready to Eat Pineapple (Ananas Comosus) Fruits Retailed at Eke Awka Market, Anambra State, Nigeria

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Isolation and Identification of Bacteria from Peeled and Ready to Eat Pineapple (Ananas Comosus) Fruits Retailed at Eke Awka Market, Anambra State, Nigeria


Umeh S. O. | Okafor O. I. | Chidubem-Nwachinemere, N. O



Umeh S. O. | Okafor O. I. | Chidubem-Nwachinemere, N. O "Isolation and Identification of Bacteria from Peeled and Ready to Eat Pineapple (Ananas Comosus) Fruits Retailed at Eke Awka Market, Anambra State, Nigeria" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-5, August 2021, pp.1237-1242, URL: https://www.ijtsrd.com/papers/ijtsrd45050.pdf

Pineapple (Ananas comosus) is an indispensible fruit that is cherished by many people due to its huge health benefits. It is peeled and sold in many markets and road sides for easy accessibility. The presence of bacteria in the peeled and ready to eat fruits was checked in this study. Peeled, sliced and cellophane packaged pineapple fruits were purchased from Eke Awka Market in Anambra State Nigeria. Nutrient agar was used to carry out bacterial isolation using pour plate technique. Results showed that colony count of the pineapple fruits ranged from 3.5 - 9.5-2cfu/ml of the rinsed water. The isolates were identified on the basis of their colony and morphological features as well as biochemical and sugar fermentation tests. Gene sequencing was used to confirm the species of some of the isolates. A total of six bacteria species were isolated and identified with frequencies as Streptococcus spp (13.9%), Pseudomonas aeruginosa (22.2%), Staphylococcus aureus (25.0%), Micrococcus luteus (11.1%), Escherichia coli (19.5%) and Staphylococcus epidermidis (8.3%). Staphylococcus aureus has the highest frequency followed by Pseudomonas aeruginosa. Staphylococcus epidermidis has the least frequency. Almost all the isolates are pathogenic in nature and their presence in the consumable fruits indicates possible health problems to the consumers. The presence of E. coli indicates direct or indirect fecal contamination. Proper handling of pineapple fruits, hygiene and proper storage will help reduce the risk of contamination by these organisms.

Spoilage, Pineapple, fruits, Post harvest loss, Proper storage


IJTSRD45050
Volume-5 | Issue-5, August 2021
1237-1242
IJTSRD | www.ijtsrd.com | E-ISSN 2456-6470
Copyright © 2019 by author(s) and International Journal of Trend in Scientific Research and Development Journal. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0) (http://creativecommons.org/licenses/by/4.0)

International Journal of Trend in Scientific Research and Development - IJTSRD having online ISSN 2456-6470. IJTSRD is a leading Open Access, Peer-Reviewed International Journal which provides rapid publication of your research articles and aims to promote the theory and practice along with knowledge sharing between researchers, developers, engineers, students, and practitioners working in and around the world in many areas like Sciences, Technology, Innovation, Engineering, Agriculture, Management and many more and it is recommended by all Universities, review articles and short communications in all subjects. IJTSRD running an International Journal who are proving quality publication of peer reviewed and refereed international journals from diverse fields that emphasizes new research, development and their applications. IJTSRD provides an online access to exchange your research work, technical notes & surveying results among professionals throughout the world in e-journals. IJTSRD is a fastest growing and dynamic professional organization. The aim of this organization is to provide access not only to world class research resources, but through its professionals aim to bring in a significant transformation in the real of open access journals and online publishing.

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