Isolation and Molecular Characterization of Pullulanase Producing Bacillus Strains

Ogbo, F. C; Nwozor, N. C

Title:
Isolation and Molecular Characterization of Pullulanase Producing Bacillus Strains

Authors:
Nwozor, N. C | Ogbo, F. C

Cite This Article :

Nwozor, N. C | Ogbo, F. C "Isolation and Molecular Characterization of Pullulanase Producing Bacillus Strains" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-5, August 2021, pp.1243-1248, URL: https://www.ijtsrd.com/papers/ijtsrd45051.pdf

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Abstract :
Pullulanase is an extracellular carbohydrase responsible for the hydrolysis of pullulan and amylopectin toproduce maltotriose. The product maltotriose is used in detergent industry, bakery industry and in the production of biotechnological products. In the present investigation pullulanase producing bacillus species were isolated and characterized using different biochemical and molecular methodologies. The isolates were identified as Bacillus cereus and Bacillus thuringiensis respectively.. The pullulanase acivity was higher in Bacillus cereus, 0.62U/ml than B. thuringiensis, 0.53 U/ml. This research reveals that pullulanase enzyme production from these Bacillus species shows great promise for use in industrial processes.

Keywords :
Pullulanase, Bacillus, Molecular, Characterization

Publication Details:

Unique Identification Number : IJTSRD45051

Published In : Volume-5 | Issue-5, August 2021

Page Number(s) : 1243-1248

Publisher Name : IJTSRD | www.ijtsrd.com | E-ISSN 2456-6470

Copyright © 2019 by author(s) and International Journal of Trend in Scientific Research and Development Journal. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0) (http://creativecommons.org/licenses/by/4.0)

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