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An Improved Slide Culture Technique for the Microscopic Identification of Fungal Species

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An Improved Slide Culture Technique for the Microscopic Identification of Fungal Species


Agu, Kingsley Chukwuebuka | Chidozie, Chiamaka Perpetua



Agu, Kingsley Chukwuebuka | Chidozie, Chiamaka Perpetua "An Improved Slide Culture Technique for the Microscopic Identification of Fungal Species" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-6 | Issue-1, December 2021, pp.243-254, URL: https://www.ijtsrd.com/papers/ijtsrd45058.pdf

Several phyto-pathogenic fungi have been discovered by numerous researchers who continue to be saddled with the problem of proper identification of these fungal agents. The conventional method requires plating out the diseased tissues of such plant materials onto culture media and observing their gross morphological features on agar plates. As soon as their colonial characteristics have been studied, the microscopic examination of fungal reproductive structures (spores) and mycelia must be done as a confirmative method of identification which must be followed by Molecular identification in order to ensure complete identification. This step often disturbs the fragile spore-hypha arrangements, thus, leading difficulties in interpretation of morphological results owing to the teasing effect in the preparation of wet mounts. The slide culture method of identification developed by Riddel in 1950 which uses an agar block of medium transferred to a glass slide and put in a moist petri dish have since been used with various modifications that have not really taken care of the slight disturbance of the mycelial arrangement of the study fungi that occurs during removal and replacement of coverslips during lactophenol cotton blue stain. We have developed a rapid slide-culture method that can diminsh this problem to the barest minimum.

Slide Culture, Microscopy, Pathogen, Fungal Species


IJTSRD45058
Volume-6 | Issue-1, December 2021
243-254
IJTSRD | www.ijtsrd.com | E-ISSN 2456-6470
Copyright © 2019 by author(s) and International Journal of Trend in Scientific Research and Development Journal. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0) (http://creativecommons.org/licenses/by/4.0)

International Journal of Trend in Scientific Research and Development - IJTSRD having online ISSN 2456-6470. IJTSRD is a leading Open Access, Peer-Reviewed International Journal which provides rapid publication of your research articles and aims to promote the theory and practice along with knowledge sharing between researchers, developers, engineers, students, and practitioners working in and around the world in many areas like Sciences, Technology, Innovation, Engineering, Agriculture, Management and many more and it is recommended by all Universities, review articles and short communications in all subjects. IJTSRD running an International Journal who are proving quality publication of peer reviewed and refereed international journals from diverse fields that emphasizes new research, development and their applications. IJTSRD provides an online access to exchange your research work, technical notes & surveying results among professionals throughout the world in e-journals. IJTSRD is a fastest growing and dynamic professional organization. The aim of this organization is to provide access not only to world class research resources, but through its professionals aim to bring in a significant transformation in the real of open access journals and online publishing.

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