Analytical Methods Development and Validation of Naproxen and Sumatriptan by RP HPLC

Prapulla Putta

Title:
Analytical Methods Development and Validation of Naproxen and Sumatriptan by RP HPLC

Authors:
Prapulla Putta
DOI:
https://doi.org/10.31142/ijtsrd23582

Cite This Article :

Prapulla Putta "Analytical Methods Development and Validation of Naproxen and Sumatriptan by RP HPLC" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-4, June 2019, pp.325-327, URL: https://www.ijtsrd.com/papers/ijtsrd23582.pdf

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Abstract :
Aim and objective: A simple, précised, accurate method was developed for the estimation of Crizotinib by RP-HPLC technique. Chromatographic conditions used are stationary phase BDS 250x4.6 mm, 5µ. Mobile phase buffer: Methodology: Acetonitrile in the ratio of 60;40 and flow rate was maintained at1ml/min, detection wave length was 267 nm, column temperature was set to 30o C and diluents was methanol: water System suitability parameters were studied by injecting the standard five times and results were well (50:50), Results & Discussion: Conditions were finalized as optimized method under acceptance criteria. Linearity study was carried out between 25 % to 150 % levels, r2 value was found to be as 0.999. Precision was found to be 1.26 for repeatability and 0.93 for intermediate precision. LOD and LOQ are 0.080 ug/ml. By using above method assay of marketed formulation was carried out 100.24 % was present.

Keywords :
Crizotinib, HPLC, ICH Guidelines, Method development

Publication Details:

Unique Identification Number : IJTSRD23582

Published In : Volume-3 | Issue-4, June 2019

Page Number(s) : 325-327

Publisher Name : IJTSRD | www.ijtsrd.com | E-ISSN 2456-6470

Copyright © 2019 by author(s) and International Journal of Trend in Scientific Research and Development Journal. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0) (http://creativecommons.org/licenses/by/4.0)

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