A new, simple, specific, sensitive, rapid, accurate and precise RP-HPLC method was developed for the estimation of Etodolac (ETO) and Thiocolchicoside (THIO) in bulk and combined pharmaceutical dosage forms. Etodolac (ETO) and Thiocolchicoside (THIO) was chromatographed on a Symmetry ODS C18 (4.6mm×250mm, 5µm) Column in a mobile phase consisting of Acetonitrile: Methanol: Acetate Buffer (25:20:55% v/v). The mobile phase was pumped at a flow rate of 1.0 ml/min with detection at 238 nm. The detector response was linear in the concentration of 200-600µg/ml 20-60µg/ml for Etodolac and Thiocolchicoside respectively. The intra and inter day variation was found to be less than 2%. The mean recovery of the drug from the solution was 99.98%. The developed method was validated for sensitivity, accuracy, precision, ruggedness and robustness. The % RSD results for precision and intermediate precision found less than 2.0%. The LOD and LOQ were found to be 0.86 and 1.18 µg/ml for Etodolac and 2.4 and 3.54µg/ml for Thiocolchicoside respectively. The proposed method is simple, fast, accurate, precise and reproducible hence; it can be applied for routine quality control analysis of Etodolac (ETO) and Thiocolchicoside (THIO) in bulk and combined pharmaceutical formulations.
: Accuracy, Etodolac, Precision, RP-HPLC, Thiocolchicoside and Validation
International Journal of Trend in Scientific Research and Development - IJTSRD having
online ISSN 2456-6470. IJTSRD is a leading Open Access, Peer-Reviewed International
Journal which provides rapid publication of your research articles and aims to promote
the theory and practice along with knowledge sharing between researchers, developers,
engineers, students, and practitioners working in and around the world in many areas
like Sciences, Technology, Innovation, Engineering, Agriculture, Management and
many more and it is recommended by all Universities, review articles and short communications
in all subjects. IJTSRD running an International Journal who are proving quality
publication of peer reviewed and refereed international journals from diverse fields
that emphasizes new research, development and their applications. IJTSRD provides
an online access to exchange your research work, technical notes & surveying results
among professionals throughout the world in e-journals. IJTSRD is a fastest growing
and dynamic professional organization. The aim of this organization is to provide
access not only to world class research resources, but through its professionals
aim to bring in a significant transformation in the real of open access journals
and online publishing.