A simple, sensitive, rapid and reproducible HPLC method has been developed and validated for Calibration determination of Valsartan in bulk and in pharmaceutical formulation by applying Quality intentionally. For development of HPLC method for Valsartan various trials are performed by using Design Expert software by applying 3 level factorial designs. Quantitative method development by optimization from trials intentionally Expert software. The Optimized method Desirabilityis0.998 for Mobile Phase ACN: Phosphate Buffer (65:35) in3.5 pH at maximum Wavelength 274nm at column oven temperature 40°C. The flow of mobile phase was adjusted 1.0 ml/min. and therefore the injection volume 10 µl. Optimised Standard curve showed a parametric statistic is 0.998. Retention time was found to be 4.6 min. the half of recovery was found to be within the bounds of the acceptance criteria with average recovery of 99.4 you take care of Valsartan. The tactic was validated as per ICH guidelines. The precision and repeatability results showed % RSD but 2%. The developed method was successfully validated in consistent with ICH guidelines. Hence, these methods are often conveniently adopted for the routine analysis in internal control laboratories.
Quality intentionally, Valsartan, HPLC, Design Expert Software
International Journal of Trend in Scientific Research and Development - IJTSRD having
online ISSN 2456-6470. IJTSRD is a leading Open Access, Peer-Reviewed International
Journal which provides rapid publication of your research articles and aims to promote
the theory and practice along with knowledge sharing between researchers, developers,
engineers, students, and practitioners working in and around the world in many areas
like Sciences, Technology, Innovation, Engineering, Agriculture, Management and
many more and it is recommended by all Universities, review articles and short communications
in all subjects. IJTSRD running an International Journal who are proving quality
publication of peer reviewed and refereed international journals from diverse fields
that emphasizes new research, development and their applications. IJTSRD provides
an online access to exchange your research work, technical notes & surveying results
among professionals throughout the world in e-journals. IJTSRD is a fastest growing
and dynamic professional organization. The aim of this organization is to provide
access not only to world class research resources, but through its professionals
aim to bring in a significant transformation in the real of open access journals
and online publishing.